Comparative study of isolated Guggulsterones as marker compounds from Guggulu, Commiphora mukul with Ayurvedic Gugglu containing formulations by HPTLC, an in-house quality control method

Selvakumar Duraipandi, Vijaya Selvakumar


The guggulu containing polyherbal formulations in Ayurveda have been used for treating various inflammatory conditions. A simple HPTLC method has been developed to qualitatively analyze the formulations that claimed to have contained guggulu using the Guggulsterones isolated from the guggulu raw material as a marker. The isolation of Guggulsterones from the resinous gum obtained from the plant was used as a marker to determine the Guggulsterone content in the formulations. The study showed that all the preparations taken for analysis that claimed to contain Guggulsterones was originally having the contents but in variable amounts depending on the amount of resin taken for the preparation. Due to the fact that Guggulsterones are very expensive marker compounds if procured separately as Guggulsterone E and Z forms, this method can be used for routine qualitative analysis of presence of Guggulsterones as an in-house quality control method.


guggulsterones; HPTLC; guggulu(Gulgulu); ayurveda

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Ayurvedic Pharmacopoeia of India, Part II (Formulations). 1 ed.; Government of India: New Delhi, 2007; Vol. 1.

Badmaev, V.; Majeed, M.; Pacchetti, B.; Prakash, L., Standardiation of Commiphora Mukul extract in dislipidemia and cardiovascular disease. NUTRA Foods 2003, 2, 45-51.

Soni, V., Isolation of E & Z guggulsterones from young aerial stems of Commiphora wightii without destruction of plants. Planta Medica 2006, 72, S_046.

Patel, S.; Patel, J.; Patel, K., Comparison and Quantification of Marker Compound of Triphala Guggulu by using HPTLC method. Am J PharmTech Res 2012, 2, 999-1013.

Rajani, M.; Ravishankara, M. N.; Shrivastava, N.; Padh, H., HPTLC-aided phytochemical fingerprinting analysis as a tool for evaluation of herbal drugs. A case study of Ushaq (ammoniacum gum). J Planar Chromatogr Mod TLC 2001, 14, 34-41.

Agrawal, H.; Kaul, N.; Paradkar, A. R.; Mahadik, K. R., HPTLC method for guggulsterone: II. Stress degradation studies on guggulsterone. J Pharm Biomed Anal 2004, 36, 23-31.

Musharraf, S. G.; Iqbal, N.; Gulzar, U.; Ali, A.; Choudhary, M. I.; Atta ur, R., Effective separation and analysis of E- and Z-guggulsterones in Commiphora mukul resin, guggulipid and their pharmaceutical product by high performance thin-layer chromatography-densitometric method. J Pharm Biomed Anal 2011, 56, 240-5.

Shelke, N.; Kuber, V.; Bhope, S.; Jadhav, R., Simultaneous HPTLC analysis of E -guggulsterone, Z -guggulsterone, 11-keto-?-boswellic acid, and 3-acetyl-11-keto-?-boswellic acid in an anti-arthritic formulation. J Planar Chromatogr Mod TLC 2011, 24, 242-7.

Jariwala, J.; Saluja, A.; Anajwala, C.; Dakhara, S., Simultaneous estimation of Guggulsterone E & Z and Tinosporaside in Jivitprada vati by HPTLC method. Int J Green Pharm 2011, 5, 113-7.




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